Description
Use the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This system is compatible with a wide range of fluorophores (see the fluorophore compatibility chart), such as StarBright™ Secondary Antibodies, ethidium bromide, SYPRO Ruby, Coomassie, and silver stains.
Features and Benefits
Sensitive detection — detects RGB and far red/near IR secondary antibodies with low fluorescence background
Multiplex fluorescent western blotting — detects up to three proteins simultaneously and eliminates the need for stripping and reprobing
Chemiluminescent western blotting — prolduces images with sensitivity matching x-ray film
Easy-to-use software — allows quantitation and analysis of gel and blot images, features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
Sample Trays and Smart Tray Technology™ — Three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
Installation/Operational Qualification Kit — Available for ensuring installation and calibration is properly executed for highly regulated laboratory environments
Product Contents
Imager with 12.1” touch-screen display plus internal computer and camera with Image Lab™ Touch Software
Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)
Image Lab Desktop Software to install on your computer
Related Products
ChemiDoc Imaging System (12003153)
ChemiDoc™ XRS+ System
PrecisionAb™ Validated Western Blotting Antibodies
Trans-Blot® Turbo™ Transfer System
Fluorescent Secondary Antibodies for Western Blotting
Fluorescent Anti-Housekeeping Protein Antibodies
Clarity and Clarity Max Western ECL Blotting Substrates
Protein Stains
Nucleic Acid Stains and Tracking Dyes
Protein Gels including TGX Stain-Free™ Gels
Related Categories
ChemiDoc and ChemiDoc XRS+ Imaging Systems
Protein Gels including TGX Stain-Free Gels
Western Blotting
Nucleic Acid Electrophoresis and Blotting
Supporting Documents
ChemiDoc MP Imaging System Brochure
ChemiDoc MP Imaging System Flier
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software User Guide
Bio-Rad® V3 Western Workflow™
Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Installation Qualification (IQ) Protocol
ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Operational Qualification (OQ) Protocol
Online Resources
Gel and Blot Imaging Systems
Western Blotting
Protein Blotting Detection and Imaging
Imaging and Analysis
Specifications
Automation Capabilities
Smart Tray Technology™
Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus
Precalibrated focus for any zoom setting or sample height
Auto-exposure
Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding
Dynamic; precalibrated and optimized for each application
Hardware
Touch-screen functionality
Multitouch capability (2 points) 12.1″ display
Maximum image area (W x H)
21 x 16.8 cm
Detector
Cooled CCD, 6 megapixels
Dynamic range
>4 orders of magnitude
Illumination sources
Trans-UV, 302 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450–490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460–490 nm excitation
Epi-green, 520–545 nm excitation
Epi-red, 625–650 nm excitation
Epi-far red, 650–675 nm excitation
Epi-near IR, 755–777 nm excitation
Filter and application
590/110 nm standard filter to perform protein and DNA gel and blot imaging
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Data output
16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight
35 kg (78 lb)
Instrument size (L x W x H)
61 x 51 x 53 cm
Operating voltage
100–250 V
Operating temperature
10–28°C
Operating humidity
10–85% relative humidity (non-condensing)
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