Real-Time Quantitative PCR System YR06248 – YR06249

Description

Description
The Real-Time Quantitative PCR System YR06248 – YR06249 is a highly advanced tool designed for precise and sensitive PCR analysis. It features cutting-edge signal capture and spectrum separation technology, ensuring excellent correction of multicolor crosstalk and consistent temperature uniformity. With a temperature range from 4°C to 100°C, and a Peltier-based heating and cooling system, it guarantees reliable results and reproducibility.
This PCR system offers both 4-channel and 5-channel configurations, making it ideal for multiplex PCR applications. It supports up to 4 targets simultaneously, or 5 targets with the 5-channel model. Compatible with a wide range of qPCR dyes and probes, including FAM, VIC, ROX, and Cy5, it is versatile enough for various lab needs. Its dynamic range spans up to 10 orders of magnitude, and it can detect as low as a single gene copy.

Advantages:
High sensitivity, capable of detecting a single gene copy.
Compatible with a wide range of dyes and probes.
Supports multiplexing up to 4 targets (5 for the 5-channel model).
High resolution and accuracy, even with complex samples.
Precision heating and cooling with Peltier technology.

Disadvantages:
High cost, which may be a concern for labs with limited budgets.
Its size could be a challenge in smaller laboratory spaces.
Real-World Use
The YR06248 – YR06249 PCR system is primarily used in genetic research labs, molecular diagnostics, and pharmaceutical studies. It is invaluable for DNA and RNA quantification, gene expression analysis, genotyping, and genetic variant studies. Clinically, it is used for diagnosing infectious diseases, cancer, and genetic disorders. The system’s multiplex PCR capability enables efficient analysis of multiple biomarkers in one run, saving both time and resources in high-demand labs.

Recommendations
To achieve optimal results, use high-quality reagents that are compatible with the system. Regular calibration of temperature and optical systems is crucial. Labs dealing with low-concentration or complex samples will particularly benefit from the system’s high precision and dynamic range.

Technical Specifications
Model YR06248 YR06249
Sample capacity 8*0.1mL PCR strip*12
96*0.1mL individual tube
0.1mL PCR Plate
Sample volume 10-50 μL
Heating and Cooling method Peltier
Maximum ramping rate 6.0° C/s
Temperature range 4-100 °C
Temperature accuracy ± 0.2°C
Temperature uniformity ±0.2°C@60°C ±0.3°C@95°C
Optical Detecting System
Excitation light 4pcs high-efficiency single color LED 5pcs high-efficiency single color LED
Detector SiPMT
Detection method Time-resolved real-time scanning
Excitation/Emission wavelengths 455-650nm 510-750nm
Detection Channels 4 (optional 5 channels）
Supported dyes FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model）
Multiplexing Up to 4 targets (optional 5 targets for 5-channel model)
Sensitivity 1 copy gene
Resolution 1.33-fold copies difference in single-plex reaction
Dynamic range 10 orders of magnitude