Aqualog – A-TEEM Industrial QC/QA Analyzer

Description

A-TEEM™ Molecular Fingerprinting is a new optical technique that is ideal for comprehensive component analysis in a variety QC/QA applications, from water to wine to pharmaceuticals and more. It is a simple, fast, “column free” spectroscopic technique that simultaneously measures the absorbance, transmission and fluorescence of samples in solution, and offers unique benefits over traditional analytical techniques, such as chromatography, mass spec, IR, etc.. Fundamentally, this technique is best applied to samples in solution that have fluorescing components, like an aromatic molecule, although it also detects non-fluorescing species via absorbance. The fluorescence EEM fingerprint technique has also been applied to solids and skin. Fluorescence is a highly sensitive technique compared to other spectroscopic techniques such as absorbance, FTIR and Raman, and the three-dimensional nature of the data provides for a better foundation for complex component analysis.
A-TEEM spectroscopy refers to the ability to simultaneously acquire Absorbance, Transmittance and a fluorescence Excitation Emission Matrix (A-TEEM) of a particular sample. HORIBA pioneered this technique with the patented Aqualog system, which combines A-TEEM spectroscopy with multichannel CCD detection to provide extremely fast results.
The A-TEEM technique requires no sample preparation, beyond possibly a simple dilution, and the cost and ease of use of an A-TEEM spectrometer allows it to be used both in the research lab as well as at-line or in-line in a production process. It’s a simpler, faster, and cheaper column-free chromatography technique for fluorescence components in solution. A-TEEM has taken fluorescence spectroscopy beyond the research lab and into the analytical industrial QC/QA environment.
HORIBA A-TEEM spectrometers can be used for fluorescence EEMs and for absorbance measurements for multi-component analysis and for transmission measurements for CIE colorimetry.

Fluorescence Hardware
Light source
Extended-UV: 150W vertically mounted xenon arc lamp
Excitation range
200 nm to upper limit of emission detector
Excitation bandpass
5 nm
Excitation monochromator
Subtractive double monochromator
Excitation gratings
1200 gr/mm, 250 nm blaze
Excitation wavelength accuracy
±1 nm
Choice of Detector
Red-extended
Emission range
250-800 nm
Emission grating
285 gr/mm; 350 nm blaze
Hardware pixel binning
0.58, 1.16, 2.32, 4.64 nm/pixel
Emission bandpass
5 nm
Emission spectrograph
Fixed, aberration-corrected 140 mm focal length
Emission detector
TE-cooled back-illuminated CCD
Emission integration time
5 ms minimum
CCD gain options
2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain
Sensitivity
Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)
Weight
32.72 kg (72 lbs)
Dimensions
LWH (618 x 435 x 336 mm); (24″ x 17″ x 13″)
scrollable
Absorbance Hardware
Scanning range
200-800 nm (UV lamp)
Bandpass
5 nm
Slew speed
Maximum 500 nm/s
Optical system Corrected single-beam
Detector Si photodiode
Wavelength accuracy
+/- 1 nm
Wavelength repeatability
+/- 0.5
Photometric accuracy
+/- 0.01 AU from 0 to 2 AU
Photometric stability <0.002 AU per hour
Photometric repeatability +/- 0.002 AU (0 to 1 AU)
Stray light
<1% measure with Kl standard